ABSTRACT
Objective To observe prespectively the healing quality in esophagogastrostomy anastomosis by layers. Methods The 12 dogs (adult male healthy hybrid dogs, group A) underwent esophagectomy with anastomosly layer. The anastomosis were observed grossly and histologic study was made on 5th, 8th, 14th, and 42th postoperative day (POD). The expression of the cytokines relating to wound healing such as epidermal growth factor(EGF), transforming growth factor ? 1(TGF ? 1) were detected (Immunohistochemistry, LsAB technique). Another 12 dogs underwent the intermittent whole layer with silk (group B). Results In group A, the stoma mucosa were in a better cooperation, soft, and had a thin scar. The count of the inflammation cells and fibroblast increased at the early period of postoperation (P